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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/12/2012 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. C.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, P. S.; GUIMARÃES, S. E. F. |
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Afiliação: |
C. S. NASCIMENTO, UFV; JANE DE OLIVEIRA PEIXOTO, CNPSA; L. L. VERARDO, UFV; C. F. CAMPOS, UFV; M. M. C. WELLER, UFV; V. R. FARIA, UFV; M. E. BOTELHO, UFV; MARTA FONSECA MARTINS, CNPGL; MARCO ANTONIO MACHADO, CNPGL; F. F. SILVA, UFV; P. S. LOPES, UFV; S. E. F. GUIMARÃES, UFV. |
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Título: |
Transcript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3315-3328. 2012. |
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DOI: |
https://doi.org/10.4238/2012.june.15.1 |
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Idioma: |
Inglês |
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Conteúdo: |
In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds. MenosIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (... Mostrar Tudo |
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Palavras-Chave: |
CDNA; Etiquetas de sequencia expressa. |
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Thesagro: |
Músculo; Presunto. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/942276/1/Transcript-profiling-of-expressed-sequence-tag-from-semimembranosus-muscle.pdf
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Marc: |
LEADER 02646naa a2200313 a 4500
001 1942276
005 2024-02-09
008 2012 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/2012.june.15.1$2DOI
100 1 $aNASCIMENTO, C. S.
245 $aTranscript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds.$h[electronic resource]
260 $c2012
520 $aIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds.
650 $aMúsculo
650 $aPresunto
653 $aCDNA
653 $aEtiquetas de sequencia expressa
700 1 $aPEIXOTO, J. de O.
700 1 $aVERARDO, L. L.
700 1 $aCAMPOS, C. F.
700 1 $aWELLER, M. M. C.
700 1 $aFARIA, V. R.
700 1 $aBOTELHO, M. E.
700 1 $aMARTINS, M. F.
700 1 $aMACHADO, M. A.
700 1 $aSILVA, F. F.
700 1 $aLOPES, P. S.
700 1 $aGUIMARÃES, S. E. F.
773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3315-3328. 2012.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 4 | |
| 2. |  | VERARDO, L. L.; NASCIMENTO, C. S.; SILVA, F. F.; GASPARINO, E.; MARTINS, M. F.; TORIYAMA, E.; FARIA, V. R.; BOTELHO, M. E.; COSTA, K. A.; LOPES, P. S.; GUIMARÃES, S. E. F. Identification and validation of differentially expressed genes from pig skeletal muscle. Journal of Animal Breeding and Genetics, v. 130, n. 5, p. 372-381, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. C.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, P. S.; GUIMARÃES, S. E. F. Transcript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds. Genetics and Molecular Research, v. 11, n. 3, p. 3315-3328. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 4. | | NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. DEL C. A.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, S. E. F.; GUIMARÂES. S. E. F. Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds. Genetics and molecular research, n. 11 (AOP), 2012. Projeto: 02.10.06.003| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 4 | |
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